Correlative Light and Electron Microscopy II

Correlative Light and Electron Microscopy II

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This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material Second of two volumes dedicated to Correlative Light and Electron microscopy (CLEM)Arrange and combine the distortion corrected images into a composite montage using GIMP (or your preferred equivalent). (8) Final ... Apply a Gaussian Blur (25a€“ 35 pixel radius), invert the color values, then apply automatic white balance to the image. It should now ... This will still require manual intervention from the user.

Title:Correlative Light and Electron Microscopy II
Publisher:Academic Press - 2014-09-30

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