Functional Genomics

Functional Genomics

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Dramatic technological advances in our understanding of biological systems, such as advances in manipulating and sequencing DNA, have led to further landmark discoveries, including the sequencing of the genomes of several prokaryotic and eukaryotic species. This, in turn, spawned a new interest in bioinformatics, structural biology, and high-throughput methods that would allow scientists to look at the response of the entire genome to physiological, pharmacological, and pathological changes. High-throughout methods, such as spotted DNA microarrays, are becoming more routine and more available, and experimentalists and theoreticians must be prepared to take advantage of them. This book aims to familiarize reader with available, reproducible protocols in the field and to introduce an audience of biologists to critically important data processing techniques. The key sections of the book focus on bench techniques that have been developed and are being routinely used in several genomics laboratories, as well as developmental design, data analysis, data display techniques, and bioinformatics.Harvest the bacteria by centrifuging the blocks for 5 min at 1500g in an Eppendorf centrifuge 5810R (Eppendorf, ... Prepare cDNA using the BioRobot 8000, or follow the Qiagen QIAprep 96 Turbo Miniprep kit protocol for manual extraction. 4.

Title:Functional Genomics
Author: Michael J. Brownstein, Arkady Khodursky
Publisher:Springer Science & Business Media - 2003-03-13

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