Molecular Embryology

Molecular Embryology

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In Molecular Embryology, expert investigators provide a comprehensive guide to the cutting-edge methods used today across the dramatically growing field of vertebrate molecular embryology. These powerful techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The major techniques of experimental molecular biology and the particular advantages of each different species are emphasized. Detailed, easy-to-follow protocols, together with relevant background information and helpful tips, optimize the methods for success. Molecular Embryology brings together in one volume all the major techniques and common experimental species needed to study the mechanisms of biological development in vertebrates. Bound to become a standard reference in this field, the book makes it possible for experienced and novice researchers alike to move between embryos of diverse vertebrate classes as their project progresses, ensuring their ability to utilize the experimental advantages of different systems to address specific developmental questions.Acetone powder is easy to make and the protocol can be found in Antibodies: A Laboratory Manual (7). Additionally ... To prepare the tissues for sectioning, dehydrate through a series of methanol (30, 50, 70, 90, and 100%), and clear with xylene or histoclear. Embed tissues in wax, and ... Spring Harbor, NY. Amphioxus 641 44 Wholemount In Situ Hybridization to Amphioxus Embryos 640 Jones and Smith.

Title:Molecular Embryology
Author: Paul T. Sharpe
Publisher:Springer Science & Business Media - 1999

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