Studies of 3-Deoxy-D-manno-octulosonate 8-phosphate Phosphatase: Mechanistic Insights and a Gene Fusion Example
Through a genomic database search, the first gene fusion example in the KDO pathway was revealed from Pseudomonas putida F1 as a fusion of kdsC and kdsB (kdsBC). This fusion gene not only uniquely clusters with other kds genes, but also resides in a capsular expression-related kps gene cluster. A combined colorimetric method was established to monitor phosphate released by KdsC and pyrophosphate released by KdsB, independently and simultaneously. The kdsBC gene was proved to encode a bifunctional enzyme displaying both KdsC and KdsB activities. The full-length bifunctional enzyme and each of its active domains were fully characterized.EV3|KKpGFPVAVRWftVBBV|RK I^C^PKASA. Loop II Figure 2-2 Sequence alignment of KdsC homologues. The secondary structure of E. coli KdsC is shown schematically above the alignment. Residues involved in the tetramerizationanbsp;...
| Title | : | Studies of 3-Deoxy-D-manno-octulosonate 8-phosphate Phosphatase: Mechanistic Insights and a Gene Fusion Example |
| Author | : | Li Yi |
| Publisher | : | ProQuest - 2009 |
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