Subcellular Biochemistry and Molecular Biology

Subcellular Biochemistry and Molecular Biology

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The Biology of Euglena, Volume IV: Subcellular Biochemistry and Molecular Biology focuses on the subcellular biochemistry and molecular biology of eukaryotic microorganisms that belong to the genus Euglena, including Euglena gracilis. It investigates enzymes and their functional location in Euglena cells, along with subcellular particles, the nucleus, the mitochondria, the chloroplast protein synthesis and chloroplast DNA, and the microbodies and lysosomes of Euglena. Organized into eight chapters, this volume begins with an overview of techniques in determining the location of enzymes and in isolating organelles in Euglena. It then proceeds with a discussion of the nucleus, its ultrastructure and macromolecules, and chromatin organization. The next chapters examine the morphology and ultrastructure of mitochondria, the morphology and biogenesis of microbodies and lysosomes, the nuclear-cytoplasmic interaction, and the structure and physicochemical properties of chloroplast DNA. The last two chapters consider the ribosomal RNAs of Euglena and the organization and activities of cytoplasmic, mitochondrial, and chloroplast ribosomes and polyribosomes, along with its polyadenylated and messenger RNA. This book will be of interest to biochemists, molecular biologists, botanists, and plant geneticists.With a French press, lyophilized cells were broken in a mixture of hexane and carbon tetrachloride to prevent leakage of enzymes from organelles. ... Use of pressure as high as 5000a€”8000 psi is too drastic (Khan and Kolattukudy, 1973a, b).

Title:Subcellular Biochemistry and Molecular Biology
Author: Dennis E. Buetow
Publisher:Elsevier - 2012-12-02

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